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Image Search Results
Journal: Advanced Science
Article Title: ROS Activated NETosis of Bone Marrow CD55 + Intermediate Mature Neutrophils Through HIF1α‐PADI4 Pathway to Initiate Bone Aging
doi: 10.1002/advs.202500046
Figure Lengend Snippet: scRNA‐seq analysis of BM neutrophils of 3‐mon male SAMP6 and SAMR1. (A) Flowchart of scRNA‐seq analysis of BM cells of 3‐mon male SAMP6 and SAMR1. (B) UMAP profile indicated cell clusters of BM cells of SAMP6 and SAMR1. (C) UMAP profile indicated SAMP6 and SAMR1 BM neutrophils contained 9 subsets. Pseudo‐time analysis (green curve) of neutrophils subsets. (D) Analysis of neutrophil differentiation–related genes in 9 subsets, and C12 was identified as the least mature and C17 as the most mature, consistent with differentiation trajectory of pseudo‐time analysis. (E) Proportion analysis of neutrophil subsets in SAMP6 and SAMR1 indicated C10 accumulation in SAMP6. (F) Violin plot showed the CXCR4 and CXCR2 expression in neutrophil subsets along with neutrophil maturation. High expression of CXCR4 and intermediate expression of CXCR2 was shown in C10. (G) Heatmap of gene expression showed CD55 significantly upregulated in C10. (H) Violin plot showed the highest expression of CD55 in C10. (I) Projection of CD55‐expression in SAMP6 BM neutrophils showed its expression concentrated in C10. (J) Pseudo‐time analysis of CD55 expression trajectory indicated it exclusively expressed in the middle of neutrophil differentiation. (K) KEGG analysis indicated NETs‐formation, ROS‐related pathway and HIF1‐related pathway significantly activated in neutrophil subset C10.
Article Snippet: Single‐cell suspensions were then loaded onto microfluidic devices and
Techniques: Expressing, Gene Expression